• TAG - Theoretical and Applied Genetics 
    Abstract Volume 101 Issue 3 (2000) pp 336-343 

    Analysis of the plastome and chondriome origin in plants regenerated after asymmetric Solanum ssp. protoplast fusions

    J. O. Rasmussen (1), A. Lössl (2), O. S. Rasmussen (1)

    (1) Department of Molecular and Structural Biology, Aarhus University, C.F.Mollers Alle Building 130, DK-8000 Aarhus, Denmark Fax: +45-86196500
    (2) Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, TU München-Weihenstephan, D-85354 Freising, Germany

  • Received: 7 October 1999 / Accepted: 3 December 1999

    Abstract Protoplasts from potato cultivars used as recipient parents were fused with irradiated protoplasts from wild Solanum donor species. Regenerated plants were analysed by RAPDs to identify hybrids. Irradiation of donor protoplasts with ionizing irradiation induced a broad range of donor nuclear DNA elimination in the asymmetric hybrids. Usage of chloroplast (cp)- and mitochondrial (mt)-specific PCR markers made it possible to trace the different origins of the cp genome in seven fusion combinations, as well as the mt genomes in two fusion combinations. Regenerated plants with recipient nucleus and plastome markers from the donors were found in six of the seven analysed fusion combinations. Protoplast fusion has generated novel mt genome combinations consisting of different portions of the mt genomes from the fusion partners. Selection of heterofusion products based on fluorescence markers is an efficient method to obtain asymmetric Solanum hybrids and cybrids from most fusion combinations. Possible models for cybrid formation are discussed. 

    Key words Potato · Mitochondria · Chloroplast · Asymmetric hybrids · Cybrids 
    ISSN: 0040-5752 (printed version)
    ISSN: 1432-2242 (electronic version)

    Online publication: August 8, 2000
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Andreas LÖSSL, 19.03.2003