In somatic fusion hybrids the degree of nuclear deviations (deviations from the parental genome addition) was evaluated by use of genomic probes, provided by C. Gebhardt, MPI Cologne.

Table: Percentage of Regenerants  with Deviating RFLP Patterns
 
 
Autoradiograph of total DNA of hybrids. Hybrid regenerants: Lanes 1-17,
          DNA-Standard ( Lane 18) and fusion parents (Lane 19-20); The Blot was performed with Eco RI cut DNA;
and hybridized with probe "GP26". The hybrid in lane 5 shows a deviation in its nuclear genome
on chromosome 2 (arrow).
Abstract of Molecular Analysis
Go to "Fusion Populations analyzed"
 

Andreas Lössl
 
Andreas LÖSSL, 15.09.1999