Cytoplasm Variation of mitochondrial expression in fusion parents and hybrids 

The mt types were not only compared at the DNA level but also at the protein level. Putative differences in the physiology of distantly related mt types could be relevant for interorganellar compatibility in somatic hybrids. In order to evaluate the different cytoplasms on their expression level, in organello translations were performed with dihaploid potato genotypes with mt genomes a, b and e.
Different mitochondrially encoded translation products.   

On mitochondrial expression level hybrids from an [ a (+)  e ] fusion (lane 1,2) could be distinguished by in-organello translation from a (+)  b ] hybrids (lane 5,6) and dihaploids (lane 3,4). 

The characteristic additional translation product of 15 kDa deriving from mt type  e  is marked by an arrowhead.  

The a (+)  e ] hybrids differed in their plastid genomes (lane 1:Cp type S, lane 2: Cp type W). 

This experiment was carried out at the Justus Liebig University in Giessen, and supported by PD. Dr. Renate Horn

No differences could be found between a and b mitochondrial expression. But in the distantly related e mitochondria an additional mitochondrial translation product of ca. 15 kDa was synthesised. It also appeared in somatic hybrids from an [a (+) e] fusion containing different plastid-mitochondrial configurations (cp type S and cp type W). Fusion hybrids between a and b did not express this protein.

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Andreas LÖSSL, 10.04.2011