Aspects of fusion combining ability of dihaploid
Solanum tuberosum L.: influence of the cytoplasm
Potato Research (1998) 41: 155-162
Frei U., Stattmann M.*, Lössl A, Wenzel G
TU München-Weihenstephan, 85350 Freising, Germany
* Bioplant GmbH, 29574 Ebstorf, Germany
Summary: In potato increasing numbers of 4x clones
originating from cell fusion of 2x clones are evaluated, and it became
obvious that besides the combined nuclear genome the composition of the
cytoplasm influences significantly the phenotypic traits of the hybrid
clones. In order to analyze the influence of the parental cytoplasm on
the success of the protoplast fusion and the regeneration of hybrids itself,
data of 50 dihaploid clones used in 74 fusion combinations were analyzed.
The majority of dihaploid breeding clones belonged to the cytoplasm types
Wa , Tb
When the mt types a, b and g
were involved, fusion combinations showed a better general combining ability
compared to incorporation of the more distantly related cytoplasms d
and e. A superiority of fusions containing
the same mt type (homofusions) over the fusion of different types could
not be proved. Whereas homofusions of cytoplasm type Wa yielded significantly
more hybrids than homofusions of the type Tb.
There are indications for a specific combining ability of the cytoplasm
types Wa and Wg.
In general the influence of the parental cytoplasm types on the fusion
combining behaviour showed to be of minor importance.
Thus the knowledge of the cytoplasm type of the fusion parents is no
suitable criteria for selecting fusion parents. It has to be seen as an
additional information in order to use the whole potential of protoplast
fusion in creating new valuable cytoplasm types.
Acknowledgements: The authors
are grateful to Mr. A. Barth, Mrs. E. Gerick, Mr. C. Kornbauer and Mrs.
Ammerseder for technical assistance. This work was supported by grants
of the BMBF under the number 0310768.
Last Update: Jan, 2010